Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-24
... problem may be minimized by treating the cleaved vector with a phosphatase before ligation to cDNA . If the double - stranded cDNA contains one or more recognition sites for the restriction enzyme , it will be cleaved and subsequently ...
... problem may be minimized by treating the cleaved vector with a phosphatase before ligation to cDNA . If the double - stranded cDNA contains one or more recognition sites for the restriction enzyme , it will be cleaved and subsequently ...
Page 8-80
A Laboratory Manual Joseph Sambrook, Tom Maniatis. PROBLEMS COMMONLY ENCOUNTERED WITH cDNA CLONING Problems commonly ... problem still persists , check the other components of the first - strand reaction , using an mRNA template whose ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. PROBLEMS COMMONLY ENCOUNTERED WITH cDNA CLONING Problems commonly ... problem still persists , check the other components of the first - strand reaction , using an mRNA template whose ...
Page 13-73
... problem is systematic ( i.e. , affects all sequencing reactions ) or whether it is template - specific . If this is not immediately obvious from a comparison of the results obtained from different templates that were se- quenced on the ...
... problem is systematic ( i.e. , affects all sequencing reactions ) or whether it is template - specific . If this is not immediately obvious from a comparison of the results obtained from different templates that were se- quenced on the ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml