Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 76
Page 8-27
... procedure is that no synthesis of the second strand of cDNA is required . Furthermore , the procedure should , in theory , allow the sequences at the 5 ' terminus of the mRNA ( which are often lost during conventional cloning procedures ) ...
... procedure is that no synthesis of the second strand of cDNA is required . Furthermore , the procedure should , in theory , allow the sequences at the 5 ' terminus of the mRNA ( which are often lost during conventional cloning procedures ) ...
Page 15-5
... procedures is the generation of a set of circularly permuted linear molecules by random double - stranded cleavage ... procedure given on pages 15.27-15.31 for conversion of double - stranded plasmid DNA to linear DNA by digestion with ...
... procedures is the generation of a set of circularly permuted linear molecules by random double - stranded cleavage ... procedure given on pages 15.27-15.31 for conversion of double - stranded plasmid DNA to linear DNA by digestion with ...
Page 1-25
... procedure , 4.26-4.28 DNA , single - stranded for DNA - sequencing reactions , 4.29- 4.30 in situ hybridization , 4.40 large - scale preparation , 4.32 for mutagenesis , 15.51-15.80 plus strand of filamentous bacterio- phage , 4.3 ...
... procedure , 4.26-4.28 DNA , single - stranded for DNA - sequencing reactions , 4.29- 4.30 in situ hybridization , 4.40 large - scale preparation , 4.32 for mutagenesis , 15.51-15.80 plus strand of filamentous bacterio- phage , 4.3 ...
Other editions - View all
Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml