Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 51
Page xxvii
... PROMOTERS The Bacteriophage λ PL Promoter 17.11 The trp - lac Promoter 17.13 The Bacteriophage T7 Promoter 17.15 EXPRESSION OF EUKARYOTIC GENES : PROMOTERS AND RIBOSOME- BINDING SITES 17.17 Preparation of a DNA Fragment for Placement ...
... PROMOTERS The Bacteriophage λ PL Promoter 17.11 The trp - lac Promoter 17.13 The Bacteriophage T7 Promoter 17.15 EXPRESSION OF EUKARYOTIC GENES : PROMOTERS AND RIBOSOME- BINDING SITES 17.17 Preparation of a DNA Fragment for Placement ...
Page 10-5
A Laboratory Manual Joseph Sambrook, Tom Maniatis. T7 promoter T7 promoter FIGURE 10.2 polycloning site SP6 promoter Clone the target sequences into a plasmid that carries promoters for bacteriophage- encoded DNA - dependent RNA ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. T7 promoter T7 promoter FIGURE 10.2 polycloning site SP6 promoter Clone the target sequences into a plasmid that carries promoters for bacteriophage- encoded DNA - dependent RNA ...
Page 10-29
... promoter . It is also important to consider the disposition of restriction sites within the template DNA and downstream from it . The 5 ′ terminus of the transcript is fixed by the bacteriophage promoter , but the 3 ' terminus is ...
... promoter . It is also important to consider the disposition of restriction sites within the template DNA and downstream from it . The 5 ′ terminus of the transcript is fixed by the bacteriophage promoter , but the 3 ' terminus is ...
Other editions - View all
Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml