Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 12-2
... proteins in recombinant libraries constructed in expression vectors . In every case , these methods involve screening the library with a ligand that binds specifically to the protein of interest . Such ligands fall into three classes ...
... proteins in recombinant libraries constructed in expression vectors . In every case , these methods involve screening the library with a ligand that binds specifically to the protein of interest . Such ligands fall into three classes ...
Page 12-2
... proteins in recombinant libraries constructed in expression vectors . In every case , these methods involve screening the library with a ligand that binds specifically to the protein of interest . Such ligands fall into three classes ...
... proteins in recombinant libraries constructed in expression vectors . In every case , these methods involve screening the library with a ligand that binds specifically to the protein of interest . Such ligands fall into three classes ...
Page 15-81
... protein and ( 2 ) X - ray diffraction of protein crystals . Although both of these methods have yielded much information , they can be used only with proteins that are available in large quantity and are of high purity . A less direct ...
... protein and ( 2 ) X - ray diffraction of protein crystals . Although both of these methods have yielded much information , they can be used only with proteins that are available in large quantity and are of high purity . A less direct ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml