Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 69
Page 10-2
... radioactive precursors ( usually 32P ) were introduced into cells that were synthesizing the DNA of interest . This procedure required large amounts of radioactivity , involved laborious purifi- cation of the nucleic acids of interest ...
... radioactive precursors ( usually 32P ) were introduced into cells that were synthesizing the DNA of interest . This procedure required large amounts of radioactivity , involved laborious purifi- cation of the nucleic acids of interest ...
Page 11-57
... radioactivity ( by Cerenkov counting , see Appendix E ) in all of the vials . Calculate the proportion of the total radioactivity that has eluted at each temperature ( i.e. , the sum of radioactivity eluted at all temperatures between ...
... radioactivity ( by Cerenkov counting , see Appendix E ) in all of the vials . Calculate the proportion of the total radioactivity that has eluted at each temperature ( i.e. , the sum of radioactivity eluted at all temperatures between ...
Page 15-67
... radioactivity at the origin . By measuring the amount of radioactivity at the origin and on the total strip , the percentage of radiolabel transferred from [ y - 32P ] ATP to the oligonucleotide can be calculated . The specific activity ...
... radioactivity at the origin . By measuring the amount of radioactivity at the origin and on the total strip , the percentage of radiolabel transferred from [ y - 32P ] ATP to the oligonucleotide can be calculated . The specific activity ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml