Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 93
Page xviii
... RADIOLABELED PROBES TO IMMOBILIZED NUCLEIC ACIDS 9.47 Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Nitrocellulose Filters or Nylon Membranes 9.52 Hybridization of Radiolabeled Oligonucleotides to Genomic DNA 9.56 ...
... RADIOLABELED PROBES TO IMMOBILIZED NUCLEIC ACIDS 9.47 Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Nitrocellulose Filters or Nylon Membranes 9.52 Hybridization of Radiolabeled Oligonucleotides to Genomic DNA 9.56 ...
Page 10-15
... radiolabel . Because the reaction is carried out under conditions that minimize exonucleolytic digestion , the radiolabeled product is not degraded to a detectable extent . If necessary , therefore , the reaction can be allowed to ...
... radiolabel . Because the reaction is carried out under conditions that minimize exonucleolytic digestion , the radiolabeled product is not degraded to a detectable extent . If necessary , therefore , the reaction can be allowed to ...
Page 11-34
... radiolabeled oligonucleotide by centrifugation at 12,000g for 20 minutes at 0 ° C in a microfuge . Using an ... Radiolabeled Oligonucleotides by Precipitation with 34 Purification of Radiolabeled Oligonucleotides by Precipitation with.
... radiolabeled oligonucleotide by centrifugation at 12,000g for 20 minutes at 0 ° C in a microfuge . Using an ... Radiolabeled Oligonucleotides by Precipitation with 34 Purification of Radiolabeled Oligonucleotides by Precipitation with.
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml