Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 82
Page 8-60
... reaction described above . To analyze the material produced in the reaction , it is necessary to carry out a small - scale parallel reaction : a . After all components of the large - scale reaction have been mixed at 4 ° C , place 2.5 ...
... reaction described above . To analyze the material produced in the reaction , it is necessary to carry out a small - scale parallel reaction : a . After all components of the large - scale reaction have been mixed at 4 ° C , place 2.5 ...
Page 13-43
... reactions using Sequenases , a preliminary step - an incorporation reaction - is included to ensure efficient incorporation of radioactive precursor in the newly synthesized DNA . Until a few years ago , chain - extension / chain ...
... reactions using Sequenases , a preliminary step - an incorporation reaction - is included to ensure efficient incorporation of radioactive precursor in the newly synthesized DNA . Until a few years ago , chain - extension / chain ...
Page 14-10
... reaction are used to generate templates for sequencing by the Sanger method : • A conventional reaction in which both priming oligonucleotides are present in vast excess . At the end of the reaction , the amplified DNA is desalted and ...
... reaction are used to generate templates for sequencing by the Sanger method : • A conventional reaction in which both priming oligonucleotides are present in vast excess . At the end of the reaction , the amplified DNA is desalted and ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml