Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 87
Page 9-58
... REMOVING PROBES FROM NITROCELLULOSE FILTERS 1. Heat several hundred milliliters of 0.05 × SSC , 0.01 м EDTA ( pH 8.0 ) to boiling . Remove the fluid from the heat and add SDS to a final concen- tration of 0.1 % . Immerse the filter in ...
... REMOVING PROBES FROM NITROCELLULOSE FILTERS 1. Heat several hundred milliliters of 0.05 × SSC , 0.01 м EDTA ( pH 8.0 ) to boiling . Remove the fluid from the heat and add SDS to a final concen- tration of 0.1 % . Immerse the filter in ...
Page 11-36
... remove all of the fluid from the tube , and repeat the washing procedure . 6. Carefully remove all of the fluid from the tube , and add 500 μl of a solution consisting of 80 % ethanol and 20 % 0.1 м sodium acetate ( pH 5.2 ) . Vortex ...
... remove all of the fluid from the tube , and repeat the washing procedure . 6. Carefully remove all of the fluid from the tube , and add 500 μl of a solution consisting of 80 % ethanol and 20 % 0.1 м sodium acetate ( pH 5.2 ) . Vortex ...
Page 13-54
... remove the shark's tooth comb from the top of the gel , and strip the electrical tape from the bottom of the gel mold . This is best done by cutting the tape into several segments with a scalpel blade and then removing each segment in ...
... remove the shark's tooth comb from the top of the gel , and strip the electrical tape from the bottom of the gel mold . This is best done by cutting the tape into several segments with a scalpel blade and then removing each segment in ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml