Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 32
Page v
... Replication and Incompatibility 1.3 Mobilization 1.5 Selectable Markers 1.5 Plasmid Vectors 1.7 DEVELOPMENT OF PLASMID CLONING VECTORS 1.7 Plasmid Vectors That Permit Histochemical Identification of Recombinant Clones 1.8 Plasmid ...
... Replication and Incompatibility 1.3 Mobilization 1.5 Selectable Markers 1.5 Plasmid Vectors 1.7 DEVELOPMENT OF PLASMID CLONING VECTORS 1.7 Plasmid Vectors That Permit Histochemical Identification of Recombinant Clones 1.8 Plasmid ...
Page 1-25
... replication , 4.3-4.6 restriction enzyme digestion analysis , 4.28 small - scale preparation analysis of recombinants , 4.41 procedure , 4.26-4.28 DNA , single - stranded for DNA - sequencing reactions , 4.29- 4.30 in situ hybridization ...
... replication , 4.3-4.6 restriction enzyme digestion analysis , 4.28 small - scale preparation analysis of recombinants , 4.41 procedure , 4.26-4.28 DNA , single - stranded for DNA - sequencing reactions , 4.29- 4.30 in situ hybridization ...
Page 1-37
... replicating bacterial colonies onto nitro- cellulose filters , 1.93-1.95 Replication of bacteriophage λ , 2.5 of bacteriophage M13 , 4.4-4.6 of plasmids , 1.2-1.5 relaxed , 1.3 rolling circle , 2.4 viral replicons , 16.8-16.9 ...
... replicating bacterial colonies onto nitro- cellulose filters , 1.93-1.95 Replication of bacteriophage λ , 2.5 of bacteriophage M13 , 4.4-4.6 of plasmids , 1.2-1.5 relaxed , 1.3 rolling circle , 2.4 viral replicons , 16.8-16.9 ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml