Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 86
Page 15-8
... restriction sites . Enzyme ( s ) should be chosen that have potential cleavage sites distributed over the entire length of the target sequence . Often , it is necessary to use two or more restriction enzymes to obtain the desired type ...
... restriction sites . Enzyme ( s ) should be chosen that have potential cleavage sites distributed over the entire length of the target sequence . Often , it is necessary to use two or more restriction enzymes to obtain the desired type ...
Page 15-9
... restriction enzyme and DNA . The eighth tube , which should not receive any restriction enzyme , is used as a control . e . Transfer all eight tubes simultaneously from the ice to a water bath set at the appropriate temperature for the ...
... restriction enzyme and DNA . The eighth tube , which should not receive any restriction enzyme , is used as a control . e . Transfer all eight tubes simultaneously from the ice to a water bath set at the appropriate temperature for the ...
Page 15-88
... restriction site carried by the synthetic hexameric linker and that contains ... enzyme that will generate a fragment of DNA carrying the kan ' gene . Purify ... restriction enzyme that cleaves that target DNA many times and generates ...
... restriction site carried by the synthetic hexameric linker and that contains ... enzyme that will generate a fragment of DNA carrying the kan ' gene . Purify ... restriction enzyme that cleaves that target DNA many times and generates ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml