Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 15-7
... restriction enzymes that cleave DNA frequently . Enzymes that generate blunt ends ( FnuDII , AluI , RsaI , and Hae III ) are particularly useful because they allow the direct addition of a large variety of synthetic linkers . However ...
... restriction enzymes that cleave DNA frequently . Enzymes that generate blunt ends ( FnuDII , AluI , RsaI , and Hae III ) are particularly useful because they allow the direct addition of a large variety of synthetic linkers . However ...
Page 15-8
... restriction sites . Enzyme ( s ) should be chosen that have potential cleavage sites distributed over the entire length of the target sequence . Often , it is necessary to use two or more restriction enzymes to obtain the desired type ...
... restriction sites . Enzyme ( s ) should be chosen that have potential cleavage sites distributed over the entire length of the target sequence . Often , it is necessary to use two or more restriction enzymes to obtain the desired type ...
Page 15-88
... enzymes ( Österlund et al . 1982 ) . Because closed circular double - stran- ded DNA binds less ethidium bromide than linear DNA , the first cleav- age by a restriction ... restriction enzyme 15.88 Site - directed Mutagenesis of Cloned DNA.
... enzymes ( Österlund et al . 1982 ) . Because closed circular double - stran- ded DNA binds less ethidium bromide than linear DNA , the first cleav- age by a restriction ... restriction enzyme 15.88 Site - directed Mutagenesis of Cloned DNA.
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml