Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-18
... room temperature , and , if necessary , pour the solution into a centrifuge tube . Add an equal volume of phenol equilibrated with 0.5 M Tris . Cl ( pH 8.0 ) ( see Appendix B ) and gently mix the two phases by slowly turning the tube ...
... room temperature , and , if necessary , pour the solution into a centrifuge tube . Add an equal volume of phenol equilibrated with 0.5 M Tris . Cl ( pH 8.0 ) ( see Appendix B ) and gently mix the two phases by slowly turning the tube ...
Page 12-19
... room temperature . The antibody solution can be stored at 4 ° C and reused several times . Sodium azide ( see Caution above ) should be added to a final concen- tration of 0.05 % to inhibit the growth of microorganisms . 13. Wash the ...
... room temperature . The antibody solution can be stored at 4 ° C and reused several times . Sodium azide ( see Caution above ) should be added to a final concen- tration of 0.05 % to inhibit the growth of microorganisms . 13. Wash the ...
Page 14-23
... room temperature . 5. Remove the microconcentrator from the centrifuge , and take off the retentate cup . Add 0.75 ml of H2O to the sample reservoir , replace the retentate cup , and recentrifuge as described in step 4 . 6. Repeat step ...
... room temperature . 5. Remove the microconcentrator from the centrifuge , and take off the retentate cup . Add 0.75 ml of H2O to the sample reservoir , replace the retentate cup , and recentrifuge as described in step 4 . 6. Repeat step ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml