Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 77
Page 13-40
... samples . 6. After all of the samples have been taken , incubate the microfuge tubes or microtiter plate for 30 minutes at 30 ° C . Add 1 μl of S1 stop mixture to each of the microfuge tubes or wells , and incubate for 10 minutes at 70 ...
... samples . 6. After all of the samples have been taken , incubate the microfuge tubes or microtiter plate for 30 minutes at 30 ° C . Add 1 μl of S1 stop mixture to each of the microfuge tubes or wells , and incubate for 10 minutes at 70 ...
Page 13-55
... samples . Excess TBE should be ejected before the next sample is drawn into the syringe and needle . • An automatic micropipettor ( 20 μl ) equipped with a standard tip . Because the tip is too large to fit between the glass plates , the ...
... samples . Excess TBE should be ejected before the next sample is drawn into the syringe and needle . • An automatic micropipettor ( 20 μl ) equipped with a standard tip . Because the tip is too large to fit between the glass plates , the ...
Page 13-94
... samples obtained by the procedures described on pages 13.88-13.93 in 100 μl of 1 m piperidine . 2. Close the tops of the tubes securely . If necessary , centrifuge the tubes briefly to deposit all of the fluid on the bottom . 3 ...
... samples obtained by the procedures described on pages 13.88-13.93 in 100 μl of 1 m piperidine . 2. Close the tops of the tubes securely . If necessary , centrifuge the tubes briefly to deposit all of the fluid on the bottom . 3 ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml