Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 95
Page xxi
... SCREENING EXPRESSION LIBRARIES CONSTRUCTED IN BACTERIOPHAGE A VECTORS 12.16 SCREENING BACTERIAL COLONIES 12.21 Preparation of Colonies for Screening 12.21 METHOD 1 12.21 METHOD 2 12.23 Processing Filters for Immunological Screening of ...
... SCREENING EXPRESSION LIBRARIES CONSTRUCTED IN BACTERIOPHAGE A VECTORS 12.16 SCREENING BACTERIAL COLONIES 12.21 Preparation of Colonies for Screening 12.21 METHOD 1 12.21 METHOD 2 12.23 Processing Filters for Immunological Screening of ...
Page 12-3
... screening of expression libraries when no nucleic acid probes are available for the gene of interest . Recently , synthetic double - stranded oligonucleotides corresponding to se- quences derived from the upstream regulatory elements of ...
... screening of expression libraries when no nucleic acid probes are available for the gene of interest . Recently , synthetic double - stranded oligonucleotides corresponding to se- quences derived from the upstream regulatory elements of ...
Page 12-15
... screening . • Demonstration that the fusion protein expressed by the clone can block the interaction between the ... screening . However , whichever method is used , it is essential to devise independent tests that do not rely on the ...
... screening . • Demonstration that the fusion protein expressed by the clone can block the interaction between the ... screening . However , whichever method is used , it is essential to devise independent tests that do not rely on the ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml