Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 11-42
... separate from it more effectively during electrophoresis . However , even when their lengths are identical , there is a good chance that the template and product strands will separate to some extent during electrophoresis since the rate ...
... separate from it more effectively during electrophoresis . However , even when their lengths are identical , there is a good chance that the template and product strands will separate to some extent during electrophoresis since the rate ...
Page 14-10
... separate pro- moter sequence . The expense of generating such long oligonucleotides can become burdensome when this method is used routinely . • A reaction in which one of the two primers is present in limiting concen- tration . After ...
... separate pro- moter sequence . The expense of generating such long oligonucleotides can become burdensome when this method is used routinely . • A reaction in which one of the two primers is present in limiting concen- tration . After ...
Page 15-11
... Separate the organic and aqueous phases by centrifugation at 12,000g for 3 minutes at 4 ° C in a microfuge . d . Carefully transfer the aqueous phase to the top of a spun column of Sephadex G - 75 . Separate the plasmid DNA from the ...
... Separate the organic and aqueous phases by centrifugation at 12,000g for 3 minutes at 4 ° C in a microfuge . d . Carefully transfer the aqueous phase to the top of a spun column of Sephadex G - 75 . Separate the plasmid DNA from the ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml