Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 78
Page 8-49
... sequence that have been synthesized in vitro . The sequence of these probes is deduced , using the genetic code , from short regions of the known amino acid sequence of the protein of interest . Because of the degeneracy of the genetic ...
... sequence that have been synthesized in vitro . The sequence of these probes is deduced , using the genetic code , from short regions of the known amino acid sequence of the protein of interest . Because of the degeneracy of the genetic ...
Page 13-14
... sequence required , and the facilities that are available . Only a minor proportion of projects involve the de novo accumulation of large tracts of virgin sequence . More often , sequencing is used to map and identify mutations ( e.g. ...
... sequence required , and the facilities that are available . Only a minor proportion of projects involve the de novo accumulation of large tracts of virgin sequence . More often , sequencing is used to map and identify mutations ( e.g. ...
Page 13-18
... sequencing project relies heavily on computer programs to sort and order the primary sequence data ( Staden 1986 ) . Access to adequate computing facilities becomes an overriding consideration when weighing the pros and cons of the ...
... sequencing project relies heavily on computer programs to sort and order the primary sequence data ( Staden 1986 ) . Access to adequate computing facilities becomes an overriding consideration when weighing the pros and cons of the ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml