Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 81
Page 11-5
... sequences of these oligonucleotides are typically deduced , using the genetic code , from short tracts of amino acid sequence determined by the sequencing of small quantities of highly purified proteins ( see , e.g. , Hewick et al ...
... sequences of these oligonucleotides are typically deduced , using the genetic code , from short tracts of amino acid sequence determined by the sequencing of small quantities of highly purified proteins ( see , e.g. , Hewick et al ...
Page 12-4
... sequences are cloned into a restriction site that lies within the coding sequences of a prokaryotic gene ( or fragment of a gene ) located immediately downstream from the promoter / ribosome - binding site . In most vectors , this gene ...
... sequences are cloned into a restriction site that lies within the coding sequences of a prokaryotic gene ( or fragment of a gene ) located immediately downstream from the promoter / ribosome - binding site . In most vectors , this gene ...
Page 13-18
... sequencing , which might generate up to 15 kb of nucleotide sequence , therefore involves • 1 day to prepare single - stranded DNA templates • 1 day of DNA sequencing • 1 day of reading primary DNA sequences and aligning them • 2 days ...
... sequencing , which might generate up to 15 kb of nucleotide sequence , therefore involves • 1 day to prepare single - stranded DNA templates • 1 day of DNA sequencing • 1 day of reading primary DNA sequences and aligning them • 2 days ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml