Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xxiii
... Sequencing Reactions 13.61 DIDEOXY - MEDIATED SEQUENCING REACTIONS USING SEQUENASES 13.65 Preparation 13.65 Sequencing Reactions 13.67 SEQUENCING DENATURED DOUBLE - STRANDED DNA TEMPLATES Sequencing of Plasmid DNAs Purified by ...
... Sequencing Reactions 13.61 DIDEOXY - MEDIATED SEQUENCING REACTIONS USING SEQUENASES 13.65 Preparation 13.65 Sequencing Reactions 13.67 SEQUENCING DENATURED DOUBLE - STRANDED DNA TEMPLATES Sequencing of Plasmid DNAs Purified by ...
Page 13-43
... reactions , in which the annealed oligonucleotide is extended and the newly synthesized chains of DNA are terminated by incorporation of ddATP , ddGTP , ddCTP , or ddTTP . In sequencing reactions using Sequenases , a preliminary step ...
... reactions , in which the annealed oligonucleotide is extended and the newly synthesized chains of DNA are terminated by incorporation of ddATP , ddGTP , ddCTP , or ddTTP . In sequencing reactions using Sequenases , a preliminary step ...
Page 13-76
... sequencing reactions yields four blank tracks All sequencing reactions on gel contain the same blank track Variation in incorporation of radiolabel from template to template Generalized high background in all four lanes makes sequence ...
... sequencing reactions yields four blank tracks All sequencing reactions on gel contain the same blank track Variation in incorporation of radiolabel from template to template Generalized high background in all four lanes makes sequence ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml