Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-32
... SINGLE - STRANDED VECTORS This method of cloning is used to generate cDNA libraries that can be conveniently screened by hybrid depletion . Unlike hybrid selection , which can only be used to screen for clones corresponding to ...
... SINGLE - STRANDED VECTORS This method of cloning is used to generate cDNA libraries that can be conveniently screened by hybrid depletion . Unlike hybrid selection , which can only be used to screen for clones corresponding to ...
Page 10-18
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Preparation of Single - stranded Probes Single - stranded probes , which consist of only one of the two complementary strands of a given sequence of nucleic acid , have a considerable ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Preparation of Single - stranded Probes Single - stranded probes , which consist of only one of the two complementary strands of a given sequence of nucleic acid , have a considerable ...
Page 15-53
... single - stranded . This can be prepared simply and efficiently by cloning the target DNA into bacteriophage M13 or into recombi- nant plasmids ( phagemids ) containing origins of replication derived from single - stranded ...
... single - stranded . This can be prepared simply and efficiently by cloning the target DNA into bacteriophage M13 or into recombi- nant plasmids ( phagemids ) containing origins of replication derived from single - stranded ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml