Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 11-50
... solution of TMACI or TEACI through a Whatman No. 1 paper . 4. Filter the solution through a nitrocellulose filter ( e.g. , Nalge , 0.45 - micron pore size ) . Store the filtered solution in dark bottles at room temperature . 5. Measure ...
... solution of TMACI or TEACI through a Whatman No. 1 paper . 4. Filter the solution through a nitrocellulose filter ( e.g. , Nalge , 0.45 - micron pore size ) . Store the filtered solution in dark bottles at room temperature . 5. Measure ...
Page 13-48
... solution 40 % acrylamide solution 75 ml 5x TBE 50 ml urea ( ultrapure ) 230 g Adjust the volume to 500 ml with deionized water , and filter the solution through a nitrocellulose filter ( e.g. , Nalge , 0.45 - micron pore size ) . The 6 ...
... solution 40 % acrylamide solution 75 ml 5x TBE 50 ml urea ( ultrapure ) 230 g Adjust the volume to 500 ml with deionized water , and filter the solution through a nitrocellulose filter ( e.g. , Nalge , 0.45 - micron pore size ) . The 6 ...
Page 13-52
... solution in a small Erlen- meyer flask . b . Place 35 ml of 6 % acrylamide / urea top solution in a 100 - ml beaker . c . Add 40 μl of 10 % ammonium persulfate to the 6 % acrylamide / urea bottom solution . Mix the solution by rapid ...
... solution in a small Erlen- meyer flask . b . Place 35 ml of 6 % acrylamide / urea top solution in a 100 - ml beaker . c . Add 40 μl of 10 % ammonium persulfate to the 6 % acrylamide / urea bottom solution . Mix the solution by rapid ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml