Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 10-14
... specific activity degrade rapidly due to radiochemical decay ( Stent and Fuerst 1960 ) . Probes whose specific activities exceed 2 × 109 cpm / μg must therefore be used without delay . Probes radiolabeled to lower specific activity ...
... specific activity degrade rapidly due to radiochemical decay ( Stent and Fuerst 1960 ) . Probes whose specific activities exceed 2 × 109 cpm / μg must therefore be used without delay . Probes radiolabeled to lower specific activity ...
Page 10-43
... specific activity can be circumvented to a large extent by using subtracted probes radiolabeled to high specific activity . SUBTRACTED PROBES RADIOLABELED TO HIGH SPECIFIC ACTIVITY In this procedure , a preparation of subtracted cDNA is ...
... specific activity can be circumvented to a large extent by using subtracted probes radiolabeled to high specific activity . SUBTRACTED PROBES RADIOLABELED TO HIGH SPECIFIC ACTIVITY In this procedure , a preparation of subtracted cDNA is ...
Page 11-41
... specific activity of the [ a - 32P ] dNTPs in the reaction . The specific activity of a dNTP whose a - phosphate has been completely substituted by 32P is approximately 9000 Ci / mmole . Preparations with lower specific activities ...
... specific activity of the [ a - 32P ] dNTPs in the reaction . The specific activity of a dNTP whose a - phosphate has been completely substituted by 32P is approximately 9000 Ci / mmole . Preparations with lower specific activities ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml