Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 19
... activity , in units ml ' , is obtained from an activity assay . Multiplying the activity by the total volume gives the total activity ( i.e. units / ml x ml units ) . = • Specific activity ( units / mg ) - the specific activity is ...
... activity , in units ml ' , is obtained from an activity assay . Multiplying the activity by the total volume gives the total activity ( i.e. units / ml x ml units ) . = • Specific activity ( units / mg ) - the specific activity is ...
Page 54
interpreted properly by comparing specific activities . The commonly used 90Sr - to - calcium ratios were subject to ... activity . Thus the 14C specific activity of organic matter in water may be used to indicate the primary ...
interpreted properly by comparing specific activities . The commonly used 90Sr - to - calcium ratios were subject to ... activity . Thus the 14C specific activity of organic matter in water may be used to indicate the primary ...
Page 461
... Specific Activity of Cholesterol ( dpm / mg ) 8000 Subject K.B. 7000 6000 5000 Plasma Free Cholesterol Plasma Total Cholesterol 4000 3000 2000 Plasma Esterified Cholesterol 1,000 900 800 700 о 2 3 5 6 7 Days After Intravenous ...
... Specific Activity of Cholesterol ( dpm / mg ) 8000 Subject K.B. 7000 6000 5000 Plasma Free Cholesterol Plasma Total Cholesterol 4000 3000 2000 Plasma Esterified Cholesterol 1,000 900 800 700 о 2 3 5 6 7 Days After Intravenous ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml