Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 10-14
... specific activity of the product depends on the amount of template added to the reaction : the lower the amount of template , the greater the specific activity . Under the reaction conditions described , radiolabeling to maximum specific ...
... specific activity of the product depends on the amount of template added to the reaction : the lower the amount of template , the greater the specific activity . Under the reaction conditions described , radiolabeling to maximum specific ...
Page 10-43
... specific for the gastrulation stage of Xenopus development ( Sargent and Dawid 1983 ) , for murine T lymphocytes ( Davis et al . 1984 ; Hedrick et al . 1984 ) , and for rat forebrain ( Rhyner et al . 1986 ) ... SPECIFIC ACTIVITY 10 43.
... specific for the gastrulation stage of Xenopus development ( Sargent and Dawid 1983 ) , for murine T lymphocytes ( Davis et al . 1984 ; Hedrick et al . 1984 ) , and for rat forebrain ( Rhyner et al . 1986 ) ... SPECIFIC ACTIVITY 10 43.
Page 11-41
... specific activity of the [ a - 32P ] dNTPs in the reaction . The specific activity of a dNTP whose a - phosphate has been completely substituted by 32P is approximately 9000 Ci / mmole . Preparations with lower specific activities ...
... specific activity of the [ a - 32P ] dNTPs in the reaction . The specific activity of a dNTP whose a - phosphate has been completely substituted by 32P is approximately 9000 Ci / mmole . Preparations with lower specific activities ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml