Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 11-21
... sterile microfuge tube , dissolve the crude oligonucleotide ( usually 1-10 nmoles ) in 1 ml of sterile , filtered H2O ( Milli - Q or equivalent ) . Vortex the solution thoroughly . The solution is often slightly cloudy because of the ...
... sterile microfuge tube , dissolve the crude oligonucleotide ( usually 1-10 nmoles ) in 1 ml of sterile , filtered H2O ( Milli - Q or equivalent ) . Vortex the solution thoroughly . The solution is often slightly cloudy because of the ...
Page 11-37
... sterile pasteur pipette . Equilibrate the slurry of Bio - Gel P - 60 supplied by the manufac- turer in 10 volumes of 10 mм Tris Cl ( pH 8.0 ) , 0.1 % SDS . Place a sterile glass - wool plug in a sterile pasteur pipette as shown in ...
... sterile pasteur pipette . Equilibrate the slurry of Bio - Gel P - 60 supplied by the manufac- turer in 10 volumes of 10 mм Tris Cl ( pH 8.0 ) , 0.1 % SDS . Place a sterile glass - wool plug in a sterile pasteur pipette as shown in ...
Page 12-21
... sterile blunt - ended forceps ( e.g. , Millipore forceps ) , lay a sterile filter , numbered side down , on a day - old LB ( or SOB ) plate containing the appropriate antibiotic . Remove the filter from the plate , invert it , and ...
... sterile blunt - ended forceps ( e.g. , Millipore forceps ) , lay a sterile filter , numbered side down , on a day - old LB ( or SOB ) plate containing the appropriate antibiotic . Remove the filter from the plate , invert it , and ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml