Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 56
Page 8-56
... stock solution at 440 nm . The molar extinction coefficient of pure ac- tinomycin D ( m.w. = 1255 ) in aqueous solution is 21,900 . The absorbance at 440 nm of a solution containing 1 mg / ml of the drug is therefore 0.182 . Stock solutions ...
... stock solution at 440 nm . The molar extinction coefficient of pure ac- tinomycin D ( m.w. = 1255 ) in aqueous solution is 21,900 . The absorbance at 440 nm of a solution containing 1 mg / ml of the drug is therefore 0.182 . Stock solutions ...
Page 10-7
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Stock Solutions Used in Nick Translation 10 × Nick - translation ... solution into small aliquots and store them at -20 ° C . 10 × Ligation buffer 0.5 M Tris Cl ( pH 7.6 ) 100 mM MgCl2 ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Stock Solutions Used in Nick Translation 10 × Nick - translation ... solution into small aliquots and store them at -20 ° C . 10 × Ligation buffer 0.5 M Tris Cl ( pH 7.6 ) 100 mM MgCl2 ...
Page 1-2
... stock solution , B.10 Ammonium hydroxide common commercial strengths , B.2 pH values of stock solutions , B.3 Ammonium persulfate , catalysis of acrylamide polymerization , 6.36 , 6.40-6.41 , 13.48 stock solution , B.10 5 ' - AMP . See ...
... stock solution , B.10 Ammonium hydroxide common commercial strengths , B.2 pH values of stock solutions , B.3 Ammonium persulfate , catalysis of acrylamide polymerization , 6.36 , 6.40-6.41 , 13.48 stock solution , B.10 5 ' - AMP . See ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml