Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-36
... stored at -70 ° C ; more frequently , they were maintained on the surfaces of nitrocellulose filters ( Hanahan and Meselson 1983 ) . In either case , the results were unsatisfactory . The libraries were difficult to preserve without ...
... stored at -70 ° C ; more frequently , they were maintained on the surfaces of nitrocellulose filters ( Hanahan and Meselson 1983 ) . In either case , the results were unsatisfactory . The libraries were difficult to preserve without ...
Page 9-49
... stored at -20 ° C . The stock solution is diluted tenfold into prehybridization buffer ( usually 6 × SSC or 6 × SSPE containing 0.5 % SDS and 100 μg / ml denatured , fragmented salmon sperm DNA ) . 50 × Denhardt's reagent contains 5 g ...
... stored at -20 ° C . The stock solution is diluted tenfold into prehybridization buffer ( usually 6 × SSC or 6 × SSPE containing 0.5 % SDS and 100 μg / ml denatured , fragmented salmon sperm DNA ) . 50 × Denhardt's reagent contains 5 g ...
Page 13-48
... stored at 4 ° C for several weeks . 6 % Acrylamide / urea top solution 40 % acrylamide solution 75 ml 5x TBE 50 ml urea ( ultrapure ) 230 g Adjust the volume to 500 ml with deionized water , and filter the solution through a ...
... stored at 4 ° C for several weeks . 6 % Acrylamide / urea top solution 40 % acrylamide solution 75 ml 5x TBE 50 ml urea ( ultrapure ) 230 g Adjust the volume to 500 ml with deionized water , and filter the solution through a ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml