Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 87
Page 8-15
... strand was a double - stranded cDNA molecule closed at the terminus corresponding to the 5 ′ terminus of the mRNA by a hairpin loop . This loop was then digested with the single - strand- specific nuclease S1 to give a double - stranded ...
... strand was a double - stranded cDNA molecule closed at the terminus corresponding to the 5 ′ terminus of the mRNA by a hairpin loop . This loop was then digested with the single - strand- specific nuclease S1 to give a double - stranded ...
Page 8-64
... Strand of cDNA The unlabeled first - strand reaction needs no further treatment before pro- ceeding to the synthesis of the second strand of cDNA . 1. Add the following reagents directly to the first - strand reaction mixture : 10 mM ...
... Strand of cDNA The unlabeled first - strand reaction needs no further treatment before pro- ceeding to the synthesis of the second strand of cDNA . 1. Add the following reagents directly to the first - strand reaction mixture : 10 mM ...
Page 8-65
... strand of cDNA and to one containing the second strand of cDNA . Incubate all four tubes for 30 minutes at 37 ° C . g . Analyze the size of first- and second - strand products by electrophoresis through an alkaline 1 % agarose gel ( see ...
... strand of cDNA and to one containing the second strand of cDNA . Incubate all four tubes for 30 minutes at 37 ° C . g . Analyze the size of first- and second - strand products by electrophoresis through an alkaline 1 % agarose gel ( see ...
Other editions - View all
Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml