Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-15
... strand of cDNA , and almost all clones of cDNA made before 1982 were obtained using this enzymatic reaction ( see , e.g. , Efstratiadis et al ... sequences immediately adjacent to the cap Construction and Analysis of cDNA Libraries 8.15.
... strand of cDNA , and almost all clones of cDNA made before 1982 were obtained using this enzymatic reaction ( see , e.g. , Efstratiadis et al ... sequences immediately adjacent to the cap Construction and Analysis of cDNA Libraries 8.15.
Page 8-30
A Laboratory Manual Joseph Sambrook, Tom Maniatis. • The synthesis of the first strand of cDNA is comparatively inefficient , since it is often difficult to incorporate sufficient dT - tailed vector in the reaction to achieve an optimal ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. • The synthesis of the first strand of cDNA is comparatively inefficient , since it is often difficult to incorporate sufficient dT - tailed vector in the reaction to achieve an optimal ...
Page 8-64
... Strand of cDNA The unlabeled first - strand reaction needs no further treatment before pro- ceeding to the synthesis of the second strand of cDNA . 1. Add the following reagents directly to the first - strand reaction mixture : 10 mM ...
... Strand of cDNA The unlabeled first - strand reaction needs no further treatment before pro- ceeding to the synthesis of the second strand of cDNA . 1. Add the following reagents directly to the first - strand reaction mixture : 10 mM ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml