Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 31
Page 13-86
... supernatant carefully and dispose of it in the appropriate waste bottle . A + G Mix 10 μl H2O 4 μl sonicated DNA 10 μl 32P - labeled DNA Chill to 0 ° C . Add 4 μl piperidine for- mate ( pH 2.0 ) and mix . Incubate 15 minutes at 37 ° C ...
... supernatant carefully and dispose of it in the appropriate waste bottle . A + G Mix 10 μl H2O 4 μl sonicated DNA 10 μl 32P - labeled DNA Chill to 0 ° C . Add 4 μl piperidine for- mate ( pH 2.0 ) and mix . Incubate 15 minutes at 37 ° C ...
Page 14-23
... supernatant , and wash the pellet with 1 ml of 70 % ethanol at room temperature , recentrifuging if necessary . Discard the supernatant , and store the open tube at room temperature until all of the ethanol has evaporated . Redissolve ...
... supernatant , and wash the pellet with 1 ml of 70 % ethanol at room temperature , recentrifuging if necessary . Discard the supernatant , and store the open tube at room temperature until all of the ethanol has evaporated . Redissolve ...
Page 15-76
... supernatant to a 500 - ml flask containing 50 ml of 2 × YT medium with 0.25 μg / ml uridine . Add 5 ml of a mid - log - phase culture of E. coli strain CJ236 ( dut ung F ' ) ( Kunkel et al . 1987 ) . Incubate the culture with vigorous ...
... supernatant to a 500 - ml flask containing 50 ml of 2 × YT medium with 0.25 μg / ml uridine . Add 5 ml of a mid - log - phase culture of E. coli strain CJ236 ( dut ung F ' ) ( Kunkel et al . 1987 ) . Incubate the culture with vigorous ...
Other editions - View all
Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml