Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 92
Page xix
... Synthesis of RNA In Vitro 10.32 Synthesis of RNA Probes Radiolabeled to High Specific Activity 10.34 Troubleshooting 10.36 SYNTHESIS OF CDNA PROBES Identification of cDNA Clones 10.38 DIFFERENTIAL SCREENING 10.38 ABSORBED PROBES 10.40 ...
... Synthesis of RNA In Vitro 10.32 Synthesis of RNA Probes Radiolabeled to High Specific Activity 10.34 Troubleshooting 10.36 SYNTHESIS OF CDNA PROBES Identification of cDNA Clones 10.38 DIFFERENTIAL SCREENING 10.38 ABSORBED PROBES 10.40 ...
Page 8-12
... synthesis , there is a competition between degradation of the mRNA and initiation of DNA synthesis . In addition , RNAase H can cleave the RNA template near the 3 ' - hydroxyl terminus of the growing DNA strand if the polymerase pauses ...
... synthesis , there is a competition between degradation of the mRNA and initiation of DNA synthesis . In addition , RNAase H can cleave the RNA template near the 3 ' - hydroxyl terminus of the growing DNA strand if the polymerase pauses ...
Page 8-29
... synthesis of the second strand . This reaction is far less efficient than replacement synthesis of the second strand . Second , the method generates cDNA libraries in plasmid vectors , which are more difficult to maintain and screen ...
... synthesis of the second strand . This reaction is far less efficient than replacement synthesis of the second strand . Second , the method generates cDNA libraries in plasmid vectors , which are more difficult to maintain and screen ...
Other editions - View all
Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml