Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 77
Page 11-55
... target . The optimal temperature for hybridization is then determined on the basis of this value . The procedure requires a cloned target sequence that is complementary ( perfectly or imperfectly , depending on the experiment ) to the ...
... target . The optimal temperature for hybridization is then determined on the basis of this value . The procedure requires a cloned target sequence that is complementary ( perfectly or imperfectly , depending on the experiment ) to the ...
Page 13-19
... target fragment are required to determine the entire sequence of both strands of the DNA . Alternatively , a single ... sequences for regions that are complementary to synthetic DNA Sequencing 13.19.
... target fragment are required to determine the entire sequence of both strands of the DNA . Alternatively , a single ... sequences for regions that are complementary to synthetic DNA Sequencing 13.19.
Page 14-32
... target sequence . Plot this ratio against the amount of control template added to each amplification reaction . From ... target se- quence in the reaction ) . Calculate the concentration of the target sequence in the original sample ...
... target sequence . Plot this ratio against the amount of control template added to each amplification reaction . From ... target se- quence in the reaction ) . Calculate the concentration of the target sequence in the original sample ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml