Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 10-34
... template DNA by digestion with a suitable restriction enzyme . Analyze an aliquot ( 100 ng ) of the digested DNA by agarose gel electrophoresis . If necessary , add more restriction enzyme and continue incubation until there is no trace ...
... template DNA by digestion with a suitable restriction enzyme . Analyze an aliquot ( 100 ng ) of the digested DNA by agarose gel electrophoresis . If necessary , add more restriction enzyme and continue incubation until there is no trace ...
Page 13-76
... template DNA Primer - binding site deleted from template High concentrations of EDTA present Incorrect primer used Problems with a specific chain - extension / chain - termination mixture Variation in template concentration Dirty templates ...
... template DNA Primer - binding site deleted from template High concentrations of EDTA present Incorrect primer used Problems with a specific chain - extension / chain - termination mixture Variation in template concentration Dirty templates ...
Page 15-57
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Hybridization of Oligonucleotides to the Template DNA and Primer Extension In the standard double - primer ... DNA 15.57 Hybridization of Oligonucleotides to the Template DNA and Primer.
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Hybridization of Oligonucleotides to the Template DNA and Primer Extension In the standard double - primer ... DNA 15.57 Hybridization of Oligonucleotides to the Template DNA and Primer.
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml