Molecular Cloning: A Laboratory Manual, Book 2 |
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Page viii
... Bacteriophage λ Growth , Purification , and DNA Extraction 2.60 PLAQUE PURIFICATION OF BACTERIOPHAGE Preparation of Plating Bacteria 2.60 Plating Bacteriophage A 2.61 Picking Bacteriophage λ Plaques 2.63 2.60 PREPARING STOCKS OF ...
... Bacteriophage λ Growth , Purification , and DNA Extraction 2.60 PLAQUE PURIFICATION OF BACTERIOPHAGE Preparation of Plating Bacteria 2.60 Plating Bacteriophage A 2.61 Picking Bacteriophage λ Plaques 2.63 2.60 PREPARING STOCKS OF ...
Page xi
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Plating Bacteriophage M13 4.22 Picking Bacteriophage M13 Plaques 4.24 PREPARING STOCKS OF BACTERIOPHAGE M13 FROM SINGLE PLAQUES 4.25 Liquid Cultures 4.25 PREPARATION OF BACTERIOPHAGE M13 ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Plating Bacteriophage M13 4.22 Picking Bacteriophage M13 Plaques 4.24 PREPARING STOCKS OF BACTERIOPHAGE M13 FROM SINGLE PLAQUES 4.25 Liquid Cultures 4.25 PREPARATION OF BACTERIOPHAGE M13 ...
Page 1-23
... bacteriophage XFIX , bacteriophage › vector map and de- scription , 2.35 , 2.37 , 2.38-2.39 . See also λ bacteriophage Agt10 , bacteriophage A vector , 2.11 , 2.41 . See also bacteriophage cloning cDNA in , 8.36-8.37 , 8.38 , 8.78 map ...
... bacteriophage XFIX , bacteriophage › vector map and de- scription , 2.35 , 2.37 , 2.38-2.39 . See also λ bacteriophage Agt10 , bacteriophage A vector , 2.11 , 2.41 . See also bacteriophage cloning cDNA in , 8.36-8.37 , 8.38 , 8.78 map ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml