Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-23
... termini with their 3 ' 5 ' exonucleolytic activities and fill in recessed 3 ' - hydroxyl termini with their polymerizing activities . The combina- tion of these activities therefore generates blunt - ended cDNA molecules , which are ...
... termini with their 3 ' 5 ' exonucleolytic activities and fill in recessed 3 ' - hydroxyl termini with their polymerizing activities . The combina- tion of these activities therefore generates blunt - ended cDNA molecules , which are ...
Page 10-51
... Termini of DNA The termini of DNA molecules can be radiolabeled enzymatically to yield molecules that may be used in nuclease - S1 mapping of RNA , as radiolabeled primers in primer - extension reactions , and as markers of defined size ...
... Termini of DNA The termini of DNA molecules can be radiolabeled enzymatically to yield molecules that may be used in nuclease - S1 mapping of RNA , as radiolabeled primers in primer - extension reactions , and as markers of defined size ...
Page 10-60
... termini of dephosphorylated DNA ( see pages 10.64-10.65 ) 10 bacteriophage T4 polynucleotide kinase buffer [ y - 32P ] ... termini = 0.5 mole of double - stranded DNA . For example : 1 mole of linear pBR322 DNA = 3.2 × 10 ° g 1 mole of 5 ...
... termini of dephosphorylated DNA ( see pages 10.64-10.65 ) 10 bacteriophage T4 polynucleotide kinase buffer [ y - 32P ] ... termini = 0.5 mole of double - stranded DNA . For example : 1 mole of linear pBR322 DNA = 3.2 × 10 ° g 1 mole of 5 ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml