Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-34
... transfer fragments of DNA from agarose gels to solid supports ( nitrocellulose filters or nylon membranes ) : 1. Capillary transfer . In the capillary transfer method ( Southern 1975 ) , DNA fragments are carried from the gel in a flow ...
... transfer fragments of DNA from agarose gels to solid supports ( nitrocellulose filters or nylon membranes ) : 1. Capillary transfer . In the capillary transfer method ( Southern 1975 ) , DNA fragments are carried from the gel in a flow ...
Page 9-36
... transfer is generally complete within 2-3 hours . Because elec- trophoretic transfer requires comparatively large electric currents , it is often difficult to maintain the electrophoresis buffer at a temperature compatible with ...
... transfer is generally complete within 2-3 hours . Because elec- trophoretic transfer requires comparatively large electric currents , it is often difficult to maintain the electrophoresis buffer at a temperature compatible with ...
Page 9-38
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Transfer of DNA to Nitrocellulose Filters CAPILLARY TRANSFER OF DNA TO NITROCELLULOSE FILTERS 1. After electrophoresis , transfer the gel to a glass baking dish and trim away any unused ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Transfer of DNA to Nitrocellulose Filters CAPILLARY TRANSFER OF DNA TO NITROCELLULOSE FILTERS 1. After electrophoresis , transfer the gel to a glass baking dish and trim away any unused ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml