Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 15-46
... transformation reactions rather than in a single large - scale reaction . The efficiency of transformation drops markedly when more than 200 μl of competent cells are used per reaction , probably because the timing of the heat - shock ...
... transformation reactions rather than in a single large - scale reaction . The efficiency of transformation drops markedly when more than 200 μl of competent cells are used per reaction , probably because the timing of the heat - shock ...
Page 15-72
... transformed by mutagenized phagemids ( see note to step 6 , page 15.65 ) may be screened with 2P - labeled oligonucleotide ... transformation and screening as outlined below . Note that simple restreaking of positive colonies does not ...
... transformed by mutagenized phagemids ( see note to step 6 , page 15.65 ) may be screened with 2P - labeled oligonucleotide ... transformation and screening as outlined below . Note that simple restreaking of positive colonies does not ...
Page 1-45
... Transformation of competent E. coli , 1.74-1.84 electrotransformation , 1.75 FSB buffer , 1.78 morphological , by ... Transformation of Competent E coli 1 74 Transformation of E coli by High-voltage Electroporation 75.
... Transformation of competent E. coli , 1.74-1.84 electrotransformation , 1.75 FSB buffer , 1.78 morphological , by ... Transformation of Competent E coli 1 74 Transformation of E coli by High-voltage Electroporation 75.
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml