Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xxix
... Translation of mRNAs 18.76 TRANSLATION OF RNA IN RETICULOCYTE LYSATES 18.76 Preparation of Rabbit Reticulocyte Lysate 18.77 Translation of Reticulocyte Lysates 18.79 TRANSLATION OF SYNTHETIC mRNAs IN VITRO 18.81 Synthesis of Synthetic ...
... Translation of mRNAs 18.76 TRANSLATION OF RNA IN RETICULOCYTE LYSATES 18.76 Preparation of Rabbit Reticulocyte Lysate 18.77 Translation of Reticulocyte Lysates 18.79 TRANSLATION OF SYNTHETIC mRNAs IN VITRO 18.81 Synthesis of Synthetic ...
Page 8-4
... translation systems derived from reticulocytes ( see Chapter 18 ) . Bulk mRNA purified from mammalian cells should code for a large number of polypeptides with molecular weights ranging from less than 10,000 to greater than 100,000 . An ...
... translation systems derived from reticulocytes ( see Chapter 18 ) . Bulk mRNA purified from mammalian cells should code for a large number of polypeptides with molecular weights ranging from less than 10,000 to greater than 100,000 . An ...
Page 10-10
... translation are sensitive to contaminants in agarose . Thus , DNA samples eluted from agarose gels should be carefully purified as described in Chapter 6 , pages 6.32-6.35 , before they are used in nick - translation reactions ( see ...
... translation are sensitive to contaminants in agarose . Thus , DNA samples eluted from agarose gels should be carefully purified as described in Chapter 6 , pages 6.32-6.35 , before they are used in nick - translation reactions ( see ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml