Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 81
Page 10-22
... tube at 12,000g for 1-2 seconds in a microfuge to move all of the liquid to the bottom . 4. Transfer 0.5 μl of the mixture to a microfuge tube containing 15 μl of 20 mм EDTA ( pH 8.0 ) . Store the tube on ice . 5. Add 1 μl ( 5 units ) ...
... tube at 12,000g for 1-2 seconds in a microfuge to move all of the liquid to the bottom . 4. Transfer 0.5 μl of the mixture to a microfuge tube containing 15 μl of 20 mм EDTA ( pH 8.0 ) . Store the tube on ice . 5. Add 1 μl ( 5 units ) ...
Page 11-36
... tube containing the solution of radiolabeled oligonucleotide that is to be precipi- tated . Mix well . For ... tube in a dry - ice / methanol bath until the mixture is frozen . Remove the tube from the bath , and allow the mixture to ...
... tube containing the solution of radiolabeled oligonucleotide that is to be precipi- tated . Mix well . For ... tube in a dry - ice / methanol bath until the mixture is frozen . Remove the tube from the bath , and allow the mixture to ...
Page 15-9
... tube . Mix quickly , and replace the tube on ice . d . Using another fresh pipette tip , transfer 10 μl from the second tube to the third tube . Continue this procedure until seven of the tubes contain both restriction enzyme and DNA ...
... tube . Mix quickly , and replace the tube on ice . d . Using another fresh pipette tip , transfer 10 μl from the second tube to the third tube . Continue this procedure until seven of the tubes contain both restriction enzyme and DNA ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml