Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 12-38
... ml . 2. Grow a 2 - ml culture of E. coli strain Y1089 to saturation in LB medium containing 0.2 % maltose , 50 μg / ml ampicillin , and 10 mM MgCl2 . 3. Dilute 50 μl of the saturated culture with 2 ml of LB medium containing 10 mM MgCl2 ...
... ml . 2. Grow a 2 - ml culture of E. coli strain Y1089 to saturation in LB medium containing 0.2 % maltose , 50 μg / ml ampicillin , and 10 mM MgCl2 . 3. Dilute 50 μl of the saturated culture with 2 ml of LB medium containing 10 mM MgCl2 ...
Page 15-46
... μg / ml ) . Store the remainders of the ligation mixtures at −20 ° C . 11. From the numbers of colonies obtained ... μg / ml . Incubate the plates for 18-24 hours at 37 ° C . 13. At the end of the incubation , add to each plate ...
... μg / ml ) . Store the remainders of the ligation mixtures at −20 ° C . 11. From the numbers of colonies obtained ... μg / ml . Incubate the plates for 18-24 hours at 37 ° C . 13. At the end of the incubation , add to each plate ...
Page 15-89
... μg / ml , 40 μg / ml , and 60 μg / ml , respectively . Caution : Ethidium bromide is a powerful mutagen and is moderately toxic . Follow precautions detailed in step 1h , page 15.9 . b . Add 10 units of the appropriate restriction ...
... μg / ml , 40 μg / ml , and 60 μg / ml , respectively . Caution : Ethidium bromide is a powerful mutagen and is moderately toxic . Follow precautions detailed in step 1h , page 15.9 . b . Add 10 units of the appropriate restriction ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml