Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 92
Page 8-39
... vector in the correct orientation with respect to the lacZ promoter , and only one in three of the molecules inserted in the right orientation will be in the correct reading frame to generate a fusion protein . Furthermore , because ...
... vector in the correct orientation with respect to the lacZ promoter , and only one in three of the molecules inserted in the right orientation will be in the correct reading frame to generate a fusion protein . Furthermore , because ...
Page 13-21
... vector sequences by digestion with restriction enzymes and polyacrylamide or agarose gel electrophoresis . If the target DNA and the vector are similar in size and cannot be separated by gel electrophoresis , try to find a restriction ...
... vector sequences by digestion with restriction enzymes and polyacrylamide or agarose gel electrophoresis . If the target DNA and the vector are similar in size and cannot be separated by gel electrophoresis , try to find a restriction ...
Page 1-36
... vector carrying Tn5 neor , 16.10-16.14 map , 16.12 Pseudoscreening , for adsorption of anti - E . coli antibodies ... vector carrying a reporter gene , 16.57 pSV2 - dhfr , mammalian expression vector , 16.29 map , 16.11 PSV2gpt ...
... vector carrying Tn5 neor , 16.10-16.14 map , 16.12 Pseudoscreening , for adsorption of anti - E . coli antibodies ... vector carrying a reporter gene , 16.57 pSV2 - dhfr , mammalian expression vector , 16.29 map , 16.11 PSV2gpt ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml