Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 97
... vector to vector . As more sophisticated vectors have been developed , the number and variety of useful cloning sites has increased dramatically ... DNA 9.7 VECTORS RECOMMENDED FOR CONSTRUCTION LIBRARIES OF EUKARYOTIC GENOMIC DNA 9 7.
... vector to vector . As more sophisticated vectors have been developed , the number and variety of useful cloning sites has increased dramatically ... DNA 9.7 VECTORS RECOMMENDED FOR CONSTRUCTION LIBRARIES OF EUKARYOTIC GENOMIC DNA 9 7.
Page 13-21
... DNA in a random fashion that is relatively independent of sequence , and therefore potential difficulties caused by ... vector sequences by digestion with restriction enzymes and polyacrylamide or agarose gel electrophoresis . If the target ...
... DNA in a random fashion that is relatively independent of sequence , and therefore potential difficulties caused by ... vector sequences by digestion with restriction enzymes and polyacrylamide or agarose gel electrophoresis . If the target ...
Page 13-31
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Preparation of Vector DNA 1. In a total volume of 100 μl , digest 10 μg of vector DNA ( phagemid or bacteriophage M13 replicative form DNA ) with an enzyme that cleaves once within the ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Preparation of Vector DNA 1. In a total volume of 100 μl , digest 10 μg of vector DNA ( phagemid or bacteriophage M13 replicative form DNA ) with an enzyme that cleaves once within the ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml