Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 84
Page 10-27
... vitro . Thus , when a linearized plasmid is incubated in vitro with the appropriate DNA - dependent RNA polymerase and the four rNTPs , virtually all RNA synthesis is initiated at the bacteriophage promoter . The tran- scripts , which ...
... vitro . Thus , when a linearized plasmid is incubated in vitro with the appropriate DNA - dependent RNA polymerase and the four rNTPs , virtually all RNA synthesis is initiated at the bacteriophage promoter . The tran- scripts , which ...
Page 10-31
... vitro transcription reaction need not be highly purified - crude minipreparations work well . The essential requirement is that the template be free ... Vitro The conditions for in vitro Preparation of Radiolabeled DNA and RNA Probes 10.31.
... vitro transcription reaction need not be highly purified - crude minipreparations work well . The essential requirement is that the template be free ... Vitro The conditions for in vitro Preparation of Radiolabeled DNA and RNA Probes 10.31.
Page 10-32
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Synthesis of RNA In Vitro The conditions for in vitro transcription of linearized plasmid DNA contain- ing a bacteriophage promoter are : · 40 mM Tris Cl ( pH 7.5 ) 6 mM MgCl2 2 mm ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Synthesis of RNA In Vitro The conditions for in vitro transcription of linearized plasmid DNA contain- ing a bacteriophage promoter are : · 40 mM Tris Cl ( pH 7.5 ) 6 mM MgCl2 2 mm ...
Other editions - View all
Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml