Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 11-38
... volume of 100 μl or less ) on the column . Immediately after the sample has entered the column , add 100 μl of ... volume of phenol : chloroform . Reextract the organic phase with 50 μl of 10 mM Tris Cl ( pH 8.0 ) , and combine the two ...
... volume of 100 μl or less ) on the column . Immediately after the sample has entered the column , add 100 μl of ... volume of phenol : chloroform . Reextract the organic phase with 50 μl of 10 mM Tris Cl ( pH 8.0 ) , and combine the two ...
Page 11-42
... volume as possible . It is therefore better to use as substrates radiolabeled dNTPs supplied in ethanol / water rather than those supplied in buffered aqueous solvents . Appropriate volumes of the ethanolic [ a - 32P ] dNTPs can be ...
... volume as possible . It is therefore better to use as substrates radiolabeled dNTPs supplied in ethanol / water rather than those supplied in buffered aqueous solvents . Appropriate volumes of the ethanolic [ a - 32P ] dNTPs can be ...
Page 13-31
... volume of 100 μl , digest 10 μg of vector DNA ( phagemid or bacteriophage M13 replicative form DNA ) with an enzyme ... volume of 2 μl ) ; B = 100 ng of linearized , phosphatase - treated vector ( in a volume of 2 μl ) ; and C = 100 ng ...
... volume of 100 μl , digest 10 μg of vector DNA ( phagemid or bacteriophage M13 replicative form DNA ) with an enzyme ... volume of 2 μl ) ; B = 100 ng of linearized , phosphatase - treated vector ( in a volume of 2 μl ) ; and C = 100 ng ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml