Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 16
Page 1-9
... western blotting , 18.64 Cyanogen bromide cleavage of bacterial fusion proteins , 17.28-17.30 Cytidine , structure ... western blot- ting , 18.64 D dam , gene for bacterial methylase as ge- netic marker in E. coli host strain , 2.57 dATP ...
... western blotting , 18.64 Cyanogen bromide cleavage of bacterial fusion proteins , 17.28-17.30 Cytidine , structure ... western blot- ting , 18.64 D dam , gene for bacterial methylase as ge- netic marker in E. coli host strain , 2.57 dATP ...
Page 1-29
... blotting ) , 7.37 , 7.39 . See also RNA , hybrid- ization electrophoresis of RNA denatured with formaldehyde , 7.43 ... western blotting , 18.64 capillary transfer of nucleic acids to supports , 7.49-7.50 , 9.42-9.46 hybridization ...
... blotting ) , 7.37 , 7.39 . See also RNA , hybrid- ization electrophoresis of RNA denatured with formaldehyde , 7.43 ... western blotting , 18.64 capillary transfer of nucleic acids to supports , 7.49-7.50 , 9.42-9.46 hybridization ...
Page 1-36
... Western blotting SDS - polyacrylamide gel electrophoresis of . See SDS - polyacrylamide gel electrophoresis of proteins 35S labeling for immunoprecipitation , 18.26-18.29 transfer to solid supports . See Western blotting Proteolytic ...
... Western blotting SDS - polyacrylamide gel electrophoresis of . See SDS - polyacrylamide gel electrophoresis of proteins 35S labeling for immunoprecipitation , 18.26-18.29 transfer to solid supports . See Western blotting Proteolytic ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml