Advanced Time-Correlated Single Photon Counting TechniquesIn 1984 Desmond O’Connor and David Phillips published their comprehensive book „Time-correlated Single Photon Counting“. At that time time-correlated s- gle photon counting, or TCSPC, was used primarily to record fluorescence decay functions of dye solutions in cuvettes. From the beginning, TCSPC was an am- ingly sensitive and accurate technique with excellent time-resolution. However, acquisition times were relatively slow due to the low repetition rate of the light sources and the limited speed of the electronics of the 70s and early 80s. Moreover, TCSPC was intrinsically one-dimensional, i.e. limited to the recording of the wa- form of a periodic light signal. Even with these limitations, it was a wonderful te- nique. More than 20 years have elapsed, and electronics and laser techniques have made impressive progress. The number of transistors on a single chip has approximately doubled every 18 months, resulting in a more than 1,000-fold increase in compl- ity and speed. The repetition rate and power of pulsed light sources have increased by about the same factor. |
Contents
3 | 24 |
4 | 47 |
2 | 84 |
27 | 88 |
35 | 94 |
5 | 95 |
43 | 100 |
50 | 106 |
Afterpulsing | 239 |
Linear Focused | 254 |
7 | 262 |
Beam | 278 |
3 | 302 |
6 | 315 |
61 | 319 |
8 | 323 |
Other editions - View all
Advanced Time-Correlated Single Photon Counting Techniques Wolfgang Becker No preview available - 2005 |
Advanced Time-correlated Single Photon Counting Techniques Wolfgang Becker No preview available - 2005 |
Common terms and phrases
absorption afterpulsing Alexa Fluor amplitude anode avalanche photodiodes beam cable cathode CFD threshold clock correlation dark count rate decay curves decay functions delay detection detector diode laser dynode electronics emission fibre FIFO FLIM fluores fluorescence decay fluorescence lifetime fluorescence lifetime imaging fluorophores FRET Hamamatsu input intensity interference filter interval IRF width large number laser diode laser pulse laser scanning microscopes lens lifetime imaging MCP PMTs measured molecules monochromator multiplexing noise nondescanned number of photons obtained optical system oscilloscope output peak photobleaching photocathode photodiode photomultiplier photon photon counting photon distribution photon pulses picosecond pile-up pixel preamplifier pulse width quantum efficiency quenching ratio recorded repetition rate resolution router sample scattering Sect sensitivity sequence setup shown in Fig signal period single photon spectroscopy TCSPC channel TCSPC device TCSPC module TCSPC system technique time-resolved tion tissue trigger two-photon excitation typical wavelength