Understanding DNA and Gene Cloning: A Guide for the CuriousGene cloning technologies continue to spur advances in many biological disciplines. Intended for the non-scientist who is interested in gaining sufficient background to understand often complex articles, this text aims to serve as a supplement for general biology or introductory genetics courses. It is also suitable as a main text in short courses for non-science majors interested in current topics such as AIDS, biotechnology, and bioethics. The previous edition of this book was published in 1984. |
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Page 65
A Guide for the Curious Karl Drlica. 5 ' 3 ' DNA polymerase Pairs of DNA stands B g dd ddf ff 09899899 Free nucleotides Template strand Growing strand LL 5 ' Position of next nucleotide added to growing chain Figure 5-3 Function of DNA ...
A Guide for the Curious Karl Drlica. 5 ' 3 ' DNA polymerase Pairs of DNA stands B g dd ddf ff 09899899 Free nucleotides Template strand Growing strand LL 5 ' Position of next nucleotide added to growing chain Figure 5-3 Function of DNA ...
Page 66
... replication fork moves through the DNA mole- cule , it unzips the DNA and makes single strands available for replica- tion . DNA polymerase on one strand will follow the fork ( Figure 5-4 ) , continuing in the same direction as the fork .
... replication fork moves through the DNA mole- cule , it unzips the DNA and makes single strands available for replica- tion . DNA polymerase on one strand will follow the fork ( Figure 5-4 ) , continuing in the same direction as the fork .
Page 71
... DNA polymerase are outlined below to illustrate how enzymes are detected and purified . To be useful for gene cloning , enzymes must be removed from living cells . The first step of purification is to obtain a large batch of cells ...
... DNA polymerase are outlined below to illustrate how enzymes are detected and purified . To be useful for gene cloning , enzymes must be removed from living cells . The first step of purification is to obtain a large batch of cells ...
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Common terms and phrases
agar plate amino acids antibody antigen atoms bacterial cells bacterial colonies bacterial DNA bacteriophage bacterium base pairs bind biologists called carbon cDNA cellular centrifuge Chapter chemical reactions chromosome cloned genes cloning vehicle codon coli cells complementary base pairing cules culture disease DNA molecules DNA polymerase DNA replication DNA strands double-stranded encoded enzyme ERSITY Figure film gel electrophoresis gene cloning gene expression genetic engineering genetic information hemoglobin hemoglobin genes host hybridization hydrogen infection inserted introns joined lambda ligase messenger RNA molecular mRNA mutation nucleic acid nucleotide pairs nucleotide sequence occurs organisms particular phage DNA phage particles piece of DNA plaques plasmid DNA primer produce protein purified rabbit DNA radioactive probe recombinant DNA region of DNA repressor restriction endonuclease retroviruses reverse transcriptase ribosome ribozyme SAN DIEGO Scientific American single-stranded splicing structure subunits sugar tein test tube tion transcription transfer RNA transposon tumor virus viruses