Understanding DNA and Gene Cloning: A Guide for the CuriousGene cloning technologies continue to spur advances in many biological disciplines. Intended for the non-scientist who is interested in gaining sufficient background to understand often complex articles, this text aims to serve as a supplement for general biology or introductory genetics courses. It is also suitable as a main text in short courses for non-science majors interested in current topics such as AIDS, biotechnology, and bioethics. The previous edition of this book was published in 1984. |
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Page 88
... agar plate , smear the drop over the whole surface of the plate , and thus eas- ily separate the fragments . But then it would be very difficult to detect the fragments ; they are so small that they are visible only by using an electron ...
... agar plate , smear the drop over the whole surface of the plate , and thus eas- ily separate the fragments . But then it would be very difficult to detect the fragments ; they are so small that they are visible only by using an electron ...
Page 97
... agar plate . Within 20 to 30 minutes , the first infected bacteria rup- ture , releasing phage particles . So many bacteria are on the agar plate that the new viruses quickly attach to nearby bacteria and repeat the infection process ...
... agar plate . Within 20 to 30 minutes , the first infected bacteria rup- ture , releasing phage particles . So many bacteria are on the agar plate that the new viruses quickly attach to nearby bacteria and repeat the infection process ...
Page 132
... agar plate ( Figure 8-2d ) must be tested to find ones that fail to grow on ampicillin - containing agar . A piece of sterile velvet is carefully placed on the surface of the tetra- cycline - containing agar plate so it touches the ...
... agar plate ( Figure 8-2d ) must be tested to find ones that fail to grow on ampicillin - containing agar . A piece of sterile velvet is carefully placed on the surface of the tetra- cycline - containing agar plate so it touches the ...
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Common terms and phrases
agar plate amino acids antibody antigen atoms bacterial cells bacterial colonies bacterial DNA bacteriophage bacterium base pairs bind biologists called carbon cDNA cellular centrifuge Chapter chemical reactions chromosome cloned genes cloning vehicle codon coli cells complementary base pairing cules culture disease DNA molecules DNA polymerase DNA replication DNA strands double-stranded encoded enzyme ERSITY Figure film gel electrophoresis gene cloning gene expression genetic engineering genetic information hemoglobin hemoglobin genes host hybridization hydrogen infection inserted introns joined lambda ligase messenger RNA molecular mRNA mutation nucleic acid nucleotide pairs nucleotide sequence occurs organisms particular phage DNA phage particles piece of DNA plaques plasmid DNA primer produce protein purified rabbit DNA radioactive probe recombinant DNA region of DNA repressor restriction endonuclease retroviruses reverse transcriptase ribosome ribozyme SAN DIEGO Scientific American single-stranded splicing structure subunits sugar tein test tube tion transcription transfer RNA transposon tumor virus viruses