Understanding DNA and Gene Cloning: A Guide for the CuriousGene cloning technologies continue to spur advances in many biological disciplines. Intended for the non-scientist who is interested in gaining sufficient background to understand often complex articles, this text aims to serve as a supplement for general biology or introductory genetics courses. It is also suitable as a main text in short courses for non-science majors interested in current topics such as AIDS, biotechnology, and bioethics. The previous edition of this book was published in 1984. |
From inside the book
Results 1-3 of 78
Page 91
... DNA is to prepare a liquid bacte- rial culture containing billions of cells harboring plasmids ( see Chapter 2 for culture techniques ) . Each of these bacterial cells contains many copies of the circular plasmid carrying the desired DNA ...
... DNA is to prepare a liquid bacte- rial culture containing billions of cells harboring plasmids ( see Chapter 2 for culture techniques ) . Each of these bacterial cells contains many copies of the circular plasmid carrying the desired DNA ...
Page 97
... DNA replication ma- chinery . When this apparatus is in place , the phages can use nucleo- tides released from the bacterial DNA to make phage DNA . Hundreds of copies of the phage DNA are made , and within minutes other genes on the ...
... DNA replication ma- chinery . When this apparatus is in place , the phages can use nucleo- tides released from the bacterial DNA to make phage DNA . Hundreds of copies of the phage DNA are made , and within minutes other genes on the ...
Page 99
... DNA fragment is inserted into a phage DNA molecule without destroying important phage genes , the phage will reproduce the frag- ment along with its own DNA when it infects a bacterial cell . Gene cloners can determine which plaque has ...
... DNA fragment is inserted into a phage DNA molecule without destroying important phage genes , the phage will reproduce the frag- ment along with its own DNA when it infects a bacterial cell . Gene cloners can determine which plaque has ...
Other editions - View all
Common terms and phrases
agar plate amino acids antibody antigen atoms bacterial cells bacterial colonies bacterial DNA bacteriophage bacterium base pairs bind biologists called carbon cDNA cellular centrifuge Chapter chemical reactions chromosome cloned genes cloning vehicle codon coli cells complementary base pairing cules culture disease DNA molecules DNA polymerase DNA replication DNA strands double-stranded encoded enzyme ERSITY Figure film gel electrophoresis gene cloning gene expression genetic engineering genetic information hemoglobin hemoglobin genes host hybridization hydrogen infection inserted introns joined lambda ligase messenger RNA molecular mRNA mutation nucleic acid nucleotide pairs nucleotide sequence occurs organisms particular phage DNA phage particles piece of DNA plaques plasmid DNA primer produce protein purified rabbit DNA radioactive probe recombinant DNA region of DNA repressor restriction endonuclease retroviruses reverse transcriptase ribosome ribozyme SAN DIEGO Scientific American single-stranded splicing structure subunits sugar tein test tube tion transcription transfer RNA transposon tumor virus viruses