Understanding DNA and Gene Cloning: A Guide for the CuriousGene cloning technologies continue to spur advances in many biological disciplines. Intended for the non-scientist who is interested in gaining sufficient background to understand often complex articles, this text aims to serve as a supplement for general biology or introductory genetics courses. It is also suitable as a main text in short courses for non-science majors interested in current topics such as AIDS, biotechnology, and bioethics. The previous edition of this book was published in 1984. |
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Page 41
... cellular workbenches called ribosomes . It then feeds across the ribosomes , and as it does , amino acids , the subunits that make up proteins , align in the order specified by the nucleotides in the messenger RNA . During the align ...
... cellular workbenches called ribosomes . It then feeds across the ribosomes , and as it does , amino acids , the subunits that make up proteins , align in the order specified by the nucleotides in the messenger RNA . During the align ...
Page 71
... cellular components is called a cell extract . Then the enzyme of interest is separated from other cell components by a series of physical manipulations . Separa- tion is possible because every type of enzyme is physically and chemi ...
... cellular components is called a cell extract . Then the enzyme of interest is separated from other cell components by a series of physical manipulations . Separa- tion is possible because every type of enzyme is physically and chemi ...
Page 136
... cellular debris plus the cellular DNA stick tightly to the paper . The sodium hydroxide also causes the DNA to be- come single - stranded . Since the bacterial colonies had been arranged in a grid on the paper , DNA released from the ...
... cellular debris plus the cellular DNA stick tightly to the paper . The sodium hydroxide also causes the DNA to be- come single - stranded . Since the bacterial colonies had been arranged in a grid on the paper , DNA released from the ...
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Common terms and phrases
agar plate amino acids antibody antigen atoms bacterial cells bacterial colonies bacterial DNA bacteriophage bacterium base pairs bind biologists called carbon cDNA cellular centrifuge Chapter chemical reactions chromosome cloned genes cloning vehicle codon coli cells complementary base pairing cules culture disease DNA molecules DNA polymerase DNA replication DNA strands double-stranded encoded enzyme ERSITY Figure film gel electrophoresis gene cloning gene expression genetic engineering genetic information hemoglobin hemoglobin genes host hybridization hydrogen infection inserted introns joined lambda ligase messenger RNA molecular mRNA mutation nucleic acid nucleotide pairs nucleotide sequence occurs organisms particular phage DNA phage particles piece of DNA plaques plasmid DNA primer produce protein purified rabbit DNA radioactive probe recombinant DNA region of DNA repressor restriction endonuclease retroviruses reverse transcriptase ribosome ribozyme SAN DIEGO Scientific American single-stranded splicing structure subunits sugar tein test tube tion transcription transfer RNA transposon tumor virus viruses