Understanding DNA and Gene Cloning: A Guide for the CuriousGene cloning technologies continue to spur advances in many biological disciplines. Intended for the non-scientist who is interested in gaining sufficient background to understand often complex articles, this text aims to serve as a supplement for general biology or introductory genetics courses. It is also suitable as a main text in short courses for non-science majors interested in current topics such as AIDS, biotechnology, and bioethics. The previous edition of this book was published in 1984. |
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Page 77
... CLONED GENES The concepts developed in the discussion of DNA replication can now be used to add important details to the general strategy for cloning genes . DNA is first isolated from cells of interest . It is then cut in spe- cific ...
... CLONED GENES The concepts developed in the discussion of DNA replication can now be used to add important details to the general strategy for cloning genes . DNA is first isolated from cells of interest . It is then cut in spe- cific ...
Page 141
A Guide for the Curious Karl Drlica. CHAPTER NINE CLONED GENES AS RESEARCH TOOLS Gene Structure , Expression Vectors , Southern Hybridization , and Binding Proteins to DNA Overview Gene cloning technologies make it possible to obtain the ...
A Guide for the Curious Karl Drlica. CHAPTER NINE CLONED GENES AS RESEARCH TOOLS Gene Structure , Expression Vectors , Southern Hybridization , and Binding Proteins to DNA Overview Gene cloning technologies make it possible to obtain the ...
Page 150
... genes are located in the sequence by comparing the nucleotide sequence to that ex- pected from the amino acid sequence of the protein made from the gene . For ... gene P M T Origin of replication Figure 150 Cloned Genes As Research Tools.
... genes are located in the sequence by comparing the nucleotide sequence to that ex- pected from the amino acid sequence of the protein made from the gene . For ... gene P M T Origin of replication Figure 150 Cloned Genes As Research Tools.
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Common terms and phrases
agar plate amino acids antibody antigen atoms bacterial cells bacterial colonies bacterial DNA bacteriophage bacterium base pairs bind biologists called carbon cDNA cellular centrifuge Chapter chemical reactions chromosome cloned genes cloning vehicle codon coli cells complementary base pairing cules culture disease DNA molecules DNA polymerase DNA replication DNA strands double-stranded encoded enzyme ERSITY Figure film gel electrophoresis gene cloning gene expression genetic engineering genetic information hemoglobin hemoglobin genes host hybridization hydrogen infection inserted introns joined lambda ligase messenger RNA molecular mRNA mutation nucleic acid nucleotide pairs nucleotide sequence occurs organisms particular phage DNA phage particles piece of DNA plaques plasmid DNA primer produce protein purified rabbit DNA radioactive probe recombinant DNA region of DNA repressor restriction endonuclease retroviruses reverse transcriptase ribosome ribozyme SAN DIEGO Scientific American single-stranded splicing structure subunits sugar tein test tube tion transcription transfer RNA transposon tumor virus viruses